Prepared Plated Media PPM

TRYPTICASEIN SOY AGAR

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Catalog Number : 915

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Description

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TRYPTIC SOY AGAR (TSA) is a nutrient-rich general-purpose medium designed for microbiological laboratories industries, supporting the abundant growth of a wide variety of microorganisms.Some of the microorganisms that grow on this medium are the following: Streptococcus, Neisseria, Brucella, Corynebacteria, Listeria, Pasteurella, Vibrio, Haemophilus vaginalis, Candida, etc. Inoculate and incubate at 30 - 35ºC and observe after 18 - 24 hours.The European Pharmacopoeia recommends this medium in the Paragraph 2.6.12 “Microbiological examination of non-sterile products: Microbial enumeration test” for the preparation of test strain. Inoculate and incubate at 30 - 35ºC for 18 - 24 hours for Staphylococcus aureus ATCC 6538, Pseudomonas aeruginosa ATCC 9027 and Bacillus subtilis ATCC 6633. For total aerobic microbial count (growth promotion) of Staphylococcus aureus ATCC 6538, Pseudomonas aeruginosa ATCC 9027 and Bacillus subtilis ATCC 6633, inoculate ≤100 cfu/ml at 30 - 35ºC for ≤ 3 days, of and for Candida albicans ATCC 10231 and Aspergillus brasiliensis ATCC 16404 inoculate ≤100 cfu/ml at 30 - 35 ºC ≤ 5 days. For total aerobic microbial count (Suitability of counting method in the presence of the product) inoculate ≤100 cfu/ml of Staphylococcus aureus, Pseudomonas aeruginosa and Bacillus subtilis at 30 - 35ºC for ≤ 3 days, and for Candida albicans ATCC 10231 and Aspergillus brasiliensis ATCC 16404 inoculate ≤100 cfu/ml at 30 - 35 ºC ≤ 5 days.