Prepared Plated Media PPM

TRYPTICASEIN SOY BROTH (TSB)

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Catalog Number : PPM-908

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Description

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TRYPTICASEIN SOY BROTH is a medium very rich in nutrients for general use in microbiological laboratories industries. It supports the abundant growth of fastidious organisms such as pneumococci, streptococci, Neisseriae, etc. 
Containing two peptones as rich nitrogen sources, obtained by the enzymatic hydrolysis of Casein and Soy proteins, this medium supports the growth of a great variety of microorganisms, including fastidious aerobes and anaerobes. Soy peptone also contains natural sugars which promote bacterial growth. Glucose is a carbohydrate and carbon source. Sodium chloride supplies essential electrolytes for transport and osmotic balance, and Dipotassium phosphate is a buff ering agent. The European Pharmacopoeia recommends this medium in Paragraph 2.6.13 “Microbiological examination of non-Sterile products” for the test of specifi ed microorganisms, as a pre enrichment medium for sample preparations for testing of products. Inoculate and incubate at 30 - 35ºC and observe after 18 - 24 hours. In Paragraph 2.6.12 “Microbiological examination of non-sterile products: Microbial enumeration test”, it is indicated to inoculate and incubate at 30 - 35ºC ≤ 3 days for the total count of aerobics ≤100 cfu/ml. For a growth promotion test of aerobes, anaerobes and fungi, inoculate the medium with a small number of microorganisms:  no more than 100 cfu of Aspergillus brasiliensis ATCC 16404,Bacillus subtilis ATCC 6633 and Candida albicans ATCC 10231. Incubate for no more than 3 days in the case of bacteria, and no more than 5 days in the case of fungi.