Prepared Plated Media PPM

SALMONELLE CHROMOGENIC AGAR

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Catalog Number : 891

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Description

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SALMONELLE CHROMOGENIC AGAR is a selective chromogenic medium, used for the detection and presumptive identifi cation of Salmonella species from clinical samples, foods and waters. The media traditionally used to diff erentiate species of Salmonella from the rest of the Enterobacteriaceae family, based on their capacity to produce hydrogen sulfi de and their inability to ferment lactose, are not really adequate as there are more than 2000 species of Salmonella which do not have these characteristics.Casein peptone and Beef extract provide nitrogen, vitamins, minerals and amino acids essential for growth. Chromogenic mixture, in conjunction with Sodium citrate, aids in inhibiting Gram-positive organisms, Proteus and coliforms. Bacteriological agar is the solidifying agent. To identify Salmonella species, this chromogenic agent is based on the combination of two chromogenic substrates that ease quick identifi cation. These two chromogenes are X-gal and Magenta-caprylate. X-gal is a substrate incorporated to visualize the enzyme ß-D-galactosidase - producing organisms as blue green colonies. Magenta colonies are a result of the hydrolysis of Magenta-caprylate by the Salmonella species due to the inability to utilise another chromogenic substrate. Thus, non-Salmonella organisms appear blue-green or are not stained by any of the chromogenes of the medium. Inoculate with the sample and incubate at 35 ± 2°C for 18 - 24 hours.

MICROBIOLOGICAL TEST
The following results were obtained in the performance of the medium from type cultures after incubation at a temperature of 35 ± 2ºC and observed after 18 - 24 hours.